Journal: Cell Death & Disease
Article Title: CUL4B mutations impair human cortical neurogenesis through PP2A-dependent inhibition of AKT and ERK
doi: 10.1038/s41419-024-06501-3
Figure Lengend Snippet: A Representative images of sections from the day-60 cerebral organoids stained with SYN1 and MAP2. Scale bar, 20 μm. B Quantification of SYN1 + puncta density in both the isogenic control (Correct) and the patient-derived (Patient) organoids on day 60. N = 12. C Representative bright-field image of a day-45 organoid plated on a 64-electrode array in an MEA well. D Plate maps of mean firing rates of both Correct and Patient organoids on day 45 and day 60. E Representative wave forms of spiking behavior from a single electrode for each Correct or Patient organoid on day 60. F Representative raster plots of Correct and Patient organoids (day 60) over 5-min continuous recording. Quantification of mean firing rate ( G ), number of spikes ( H ), burst percentage ( I ) and burst frequency ( J ) based on data obtained from Correct and Patient organoids on day 45 and day 60 over 10 min recording. A bundle of more than 10 spikes within 100 ms was defined as a burst. N = 8 MEA wells per genotype. Data are presented as the mean ± SEM. The statistical significance was determined using two-tailed unpaired t-test. * P < 0.05; ** P < 0.01; *** P < 0.001. ns: no significance.
Article Snippet: Organoids were placed into 6-well MEA plates (Axion Biosystems, Atlanta, GA, USA, Cat# M384-tMEA-6W) coated with poly-l-ornithine/laminin (Sigma) and with 64 platinum microelectrodes arrays per well.
Techniques: Staining, Control, Derivative Assay, Two Tailed Test